Illumina MiSeq System
Short-read next-generation sequencing for amplicons, small genomes and targeted panels.
The Illumina MiSeq is a benchtop next-generation sequencer based on sequencing-by-synthesis chemistry. It produces highly accurate paired-end reads and is well suited to projects that need reliable data from a moderate number of samples.
At GML the MiSeq supports marker-gene surveys of microbial communities, small genome sequencing and targeted amplicon panels. We handle the complete workflow in house, from extraction and library preparation through quality control, sequencing and data delivery.
Applications
- 16S rRNA and ITS amplicon sequencing for microbial community analysis
- Whole-genome sequencing of bacterial isolates and small genomes
- Targeted amplicon panels and variant confirmation
- Plasmid and construct verification
- Library quality checks before larger sequencing runs
Typical Sample Types
Extracted DNA, amplicons or prepared libraries. Environmental, microbial, industrial and research samples can be extracted in house.
Key Specifications
| Chemistry | Illumina sequencing by synthesis |
|---|---|
| Read length | Up to 2 × 300 bp paired-end |
| Output | Up to approximately 15 Gb per run (v3 chemistry) |
| Reads per run | Up to approximately 25 million (v3 chemistry) |
| Data | Demultiplexed FASTQ files with run quality metrics |
Specifications are provided for general reference. Method performance depends on sample matrix and project requirements.
To discuss a project that uses the Illumina MiSeq System, please call (314) 918-5494 or submit a project quote request.
Related News
- Illumina MiSeq sequencing system commissioned – January 19, 2022
- Molecular biology services: three years of sequencing projects – September 3, 2025
- Oxford Nanopore MinION expands sequencing options – April 6, 2022
Other Molecular Biology Equipment
- Agilent 2100 Bioanalyzer
- Applied Biosystems ProFlex PCR System
- Branson 450 Digital Sonifier
- Eppendorf Mastercycler nexus GSX1
- Thermo Scientific Arktik Thermal Cycler
- Omni International Bead Ruptor 24 Elite
- Oxford Nanopore MinION with Flongle
- Qubit 2.0 Fluorometer
- Thermo Scientific PikoReal Real-Time PCR System
- UVP DigiDoc-It Imaging System