Agilent 2100 Bioanalyzer
Microfluidic sizing, quantification and quality control of DNA, RNA and protein.
The Agilent 2100 Bioanalyzer uses microfluidic chips to perform automated electrophoresis on very small sample volumes. In a single run it separates, sizes and quantifies nucleic acid or protein fragments and produces an electropherogram and gel-like image for each sample.
At GML the Bioanalyzer is the standard quality checkpoint in our molecular workflow. Extracted nucleic acids and finished sequencing libraries are checked before they move to the next step, so that degraded material or incorrect fragment sizes are caught early rather than after an expensive sequencing run.
Applications
- RNA integrity assessment (RIN) prior to gene expression or RNA-seq work
- Fragment size distribution of sequencing libraries (Illumina and Nanopore)
- Sizing and quantification of PCR products and restriction digests
- Detection of genomic DNA contamination or degradation
- Protein sizing and purity checks
Typical Sample Types
Purified DNA, total RNA, mRNA, sequencing libraries, PCR products and protein extracts. Typically 1 µL of sample is required per analysis.
Key Specifications
| Technique | Chip-based capillary electrophoresis |
|---|---|
| Sample volume | 1 µL per sample |
| Throughput | Up to 12 samples per chip (assay dependent) |
| Assays | DNA, RNA (Nano and Pico) and protein kits |
| Output | Electropherogram, virtual gel, size and concentration, RIN |
Specifications are provided for general reference. Method performance depends on sample matrix and project requirements.
To discuss a project that uses the Agilent 2100 Bioanalyzer, please call (314) 918-5494 or submit a project quote request.
Related News
- Bioanalyzer and Qubit fluorometry now available – August 25, 2021
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- Applied Biosystems ProFlex PCR System
- Branson 450 Digital Sonifier
- Eppendorf Mastercycler nexus GSX1
- Thermo Scientific Arktik Thermal Cycler
- Illumina MiSeq System
- Omni International Bead Ruptor 24 Elite
- Oxford Nanopore MinION with Flongle
- Qubit 2.0 Fluorometer
- Thermo Scientific PikoReal Real-Time PCR System
- UVP DigiDoc-It Imaging System