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Oxford Nanopore MinION with Flongle

Oxford Nanopore MinION with Flongle

Real-time long-read sequencing, including low-cost Flongle runs.

The MinION is a portable sequencer that reads single DNA or RNA molecules as they pass through protein nanopores. Unlike short-read platforms it can produce very long reads, which makes it valuable for assembling complete genomes and resolving repetitive regions.

The Flongle adapter allows smaller, lower-cost runs on a single-use flow cell. It is a practical option for plasmid verification, small amplicon projects and quick checks where a full flow cell would be unnecessary.

Applications

  • Complete bacterial genome assembly (alone or combined with MiSeq data)
  • Full-length 16S rRNA and rRNA operon sequencing
  • Plasmid and construct verification
  • Structural variant detection and resolution of repeats
  • Rapid screening and pilot projects

Typical Sample Types

High-molecular-weight DNA, amplicons and plasmid DNA. Extraction method affects achievable read length, so please consult us before sampling.

Key Specifications

TechnologyNanopore single-molecule sequencing
Read lengthDetermined by input DNA; reads over 100 kb are possible
Flow cellsMinION and Flongle
DataReal-time base calling, FASTQ output

Specifications are provided for general reference. Method performance depends on sample matrix and project requirements.

Related service: DNA Extraction, Amplification & Sequencing
To discuss a project that uses the Oxford Nanopore MinION with Flongle, please call (314) 918-5494 or submit a project quote request.

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