Thermo Scientific PikoReal Real-Time PCR System
Quantitative PCR for gene copy number and expression studies.
The PikoReal is a real-time (quantitative) PCR system that measures fluorescence during every cycle of amplification. By comparing samples against a standard curve, it determines how many copies of a target gene were present at the start of the reaction.
qPCR is often the most cost-effective way to answer a focused question, such as how abundant a particular organism or functional gene is across a set of samples, without sequencing everything.
Applications
- Absolute quantification of target genes and organisms
- Relative gene expression (RT-qPCR)
- Library quantification before sequencing
- Melt curve analysis and assay validation
- Monitoring of specific microbes in process and environmental samples
Typical Sample Types
DNA and cDNA extracts; RNA can be reverse transcribed in house.
Key Specifications
| Technique | Real-time fluorescence PCR |
|---|---|
| Chemistries | SYBR Green and probe-based (e.g. TaqMan) assays |
| Analysis | Standard curves, ΔΔCq and melt curve analysis |
| Reaction volume | Low-volume reactions to conserve samples |
Specifications are provided for general reference. Method performance depends on sample matrix and project requirements.
Related service: DNA Extraction, Amplification & Sequencing
To discuss a project that uses the Thermo Scientific PikoReal Real-Time PCR System, please call (314) 918-5494 or submit a project quote request.
To discuss a project that uses the Thermo Scientific PikoReal Real-Time PCR System, please call (314) 918-5494 or submit a project quote request.
Related News
- Real-time PCR quantification capability online – July 20, 2022
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