Size-Exclusion Chromatography (SEC) Columns, Waters ACQUITY UPLC Protein BEH SEC 125 Å
Detection of peptide aggregates, dimers and fragments by molecular size.
Size-exclusion chromatography separates molecules by size rather than by chemistry. Large species such as aggregates and dimers cannot enter the pores of the column packing and elute first; the intact peptide follows, and smaller fragments come last. The relative peak areas show how much of a sample has aggregated.
We run SEC on our UltiMate 3000 UHPLC system. Aggregation matters most for larger peptides such as GLP-1 analogues and insulin, where high-molecular-weight species can form during manufacture, shipping or storage and are not always visible in a standard reversed-phase purity test.
Applications
- Aggregate (high-molecular-weight species) analysis of GLP-1 peptides and insulin
- Dimer and oligomer detection in peptide and protein products
- Monitoring stability after storage, shipping or reconstitution
- Fragment and degradation product screening
Typical Sample Types
Peptides and proteins in solution or reconstituted from powder. Buffer and concentration are agreed per project.
Key Specifications
| Technique | Size-exclusion UHPLC with UV detection |
|---|---|
| Pore size | 125 Å |
| Particle size | 1.7 µm |
| Separation range | Approximately 1,000–80,000 Da |
| System | Thermo Scientific Dionex UltiMate 3000 UHPLC |
Specifications are provided for general reference. Method performance depends on sample matrix and project requirements.
To discuss a project that uses the Size-Exclusion Chromatography (SEC) Columns, Waters ACQUITY UPLC Protein BEH SEC 125 Å, please call (314) 652-3448 or submit a project quote request.
Other Chromatography & Mass Spectrometry Equipment
- Agilent 7890B Gas Chromatography System
- Agilent 5977A Mass Selective Detector
- Thermo Scientific Dionex ICS-5000+ Ion Chromatography System
- Thermo Scientific Dionex UltiMate 3000 UHPLC System
- Thermo Scientific Dionex UltiMate 3000 Fluorescence Detector
- Thermo Scientific Q Exactive Plus Hybrid Quadrupole-Orbitrap Mass Spectrometer
- Agilent 7900 ICP-MS
- Elementar vario EL cube CHNS Elemental Analyzer