Thermo Scientific NanoDrop 2000 Spectrophotometer
Microvolume UV-Vis measurement of nucleic acid and protein concentration and purity.
The NanoDrop 2000 measures absorbance on a sample of just 0.5–2 µL held between two optical surfaces by surface tension. No cuvette or dilution is required for most samples.
In addition to concentration, the absorbance ratios at 260/280 nm and 260/230 nm indicate whether a nucleic acid sample contains protein, phenol, salts or other carryover that could interfere with downstream work.
Applications
- Nucleic acid concentration and purity (A260/280, A260/230)
- Protein concentration at A280
- Labeling efficiency of dye-conjugated samples
- Quick checks of extraction performance
Typical Sample Types
DNA, RNA, protein and other solutions; 0.5–2 µL per measurement.
Key Specifications
| Sample volume | 0.5–2 µL |
|---|---|
| Wavelength range | 190–840 nm |
| dsDNA range | Approximately 2–15,000 ng/µL |
| Pathlength | Automatic pathlength adjustment |
Specifications are provided for general reference. Method performance depends on sample matrix and project requirements.
Related service: Analytical Chemistry
To discuss a project that uses the Thermo Scientific NanoDrop 2000 Spectrophotometer, please call (314) 918-5494 or submit a project quote request.
To discuss a project that uses the Thermo Scientific NanoDrop 2000 Spectrophotometer, please call (314) 918-5494 or submit a project quote request.
Related News
- NanoDrop and microplate reader capabilities – April 10, 2019
- Bioanalyzer and Qubit fluorometry now available – August 25, 2021
- New UV-Vis spectrophotometer installed – January 23, 2019